theIASHub | 07 Apr, 2025
RAPID-CRISPR /Acute Promyelocytic Leukemia (APL): A new test called RAPID-CRISPR, developed by ACTREC, Tata Memorial Hospital, Mumbai, using CRISPR technology, can quickly and accurately diagnose acute promyelocytic leukemia (APL) in under three hours and at lower cost than existing tests.
o It is caused by a genetic mutation where two genes, PML and RARA, mistakenly fuse together.
o This leads to the production of fewer white blood cells and platelets, reducing the body’s ability to fight infections and control bleeding.
oAPL can cause severe internal bleeding in organs like the lungs and brain, potentially resulting in death within days if untreated.
o The test delivers results in under three hours, costs less than existing tests, and does not require complex lab equipment.
o It works by cutting the PML-RARA gene and triggering a signal detected by a strip similar to a home pregnancy test.
o In RAPID-CRISPR, CRISPR is used for molecular diagnosis by detecting and cutting specific gene mutations and triggering detectable signals.
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